| Synergistic Effects of Endurance Training and Vitamin D₃ Injection on Serum BUN, Creatinine, and Renal sFas/FasL Ratio in Type 2 Diabetic Rats |
| Paper ID : 1194-SPORTCONGRESS (R1) |
| Authors |
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Alireza Aeini1, Abuzar Jorbonian *1, Hamid Arazi2, Mohammad Rahman Rahimi3, Payam Saidie1 1Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan 2Department of Exercise Physiology, Faculty of Sport Sciences, University of Ferdowsi 3Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan |
| Abstract |
| Introduction: Type 2 diabetes mellitus is a prevalent metabolic disorder frequently associated with renal dysfunction (Verzola et al., 2007). Creatinine and BUN are key markers of renal function (Gowda et al., 2010), and the sFas/FasL ratio indicates apoptosis in tissue (Huttunen et al., 2012). Endurance training and vitamin D₃ have shown potential to modulate these markers in diabetic models; however, results remain inconsistent (Golpasandi et al., 2023). This study investigates their combined effects to clarify their roles in regulating renal function and apoptosis in rats with type 2 diabetes. Methods: Forty male Wistar rats (8 weeks old, 250–300 g) were acclimated and divided into normal diet (ND; n = 8) and high-fat diet (HFD; n = 32) groups. Type 2 diabetes was induced by 6 weeks of HFD and a single Streptozotocin injection (35 mg/kg). Rats were assigned to five groups: healthy control (HC), diabetic control (DC), diabetes + endurance training (DET), diabetes + vitamin D₃ (DVD), and diabetes + vitamin D₃ + endurance training (DVDET). Training involved 8 weeks of treadmill running (55–65% MRS, 30–60 min/session), and vitamin D₃ (10,000 IU/kg) was given weekly (Golpasandi et al., 2023). Serum BUN, creatinine, and renal apoptotic markers were measured. Results: DC rats had significantly higher BUN, creatinine, and sFas/FasL ratio vs. HC (p<0.001). All three markers significantly decreased in DET, DVD, and especially DVDET (adjusted p-values: 0.034 to <0.001). No significant differences were found between DET and DVD; DVDET showed superior effects. Conclusion: Based on the findings, type 2 diabetes increases apoptosis in renal tissue. Combined application of endurance training and vitamin D₃ improved renal function and reduced apoptosis in diabetic rats. These results suggest that integrating both interventions may offer an effective strategy for ameliorating diabetes-induced renal complications. |
| Keywords |
| Apoptosis, Cholecalciferol, Diabetic Nephropathy |
| Status: Abstract Accepted (Oral Presentation) |
