| Impact of Concurrent Training Sequence on Molecular Signaling Pathway Responses in Rat Skeletal Muscle |
| Paper ID : 1215-SPORTCONGRESS |
| Authors |
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Morteza Sangdevini * عضو هیأت علمی |
| Abstract |
| Introduction: The mammalian target of rapamycin (mTOR) pathway is believed to play a major role, via phosphorylation of the ribosomal protein S6 kinase of 70 kDa (p70S6K) in hypertrophy of muscle. Its might that concurrent training interfere with mTOR signaling pathways via AMPK activation. Here, the purpose of this study was to examine the effect of 8 weeks concurrent resistance- endurance training with opposite order on mTOR, p70S6K, AMPK and MuRf-1 in rat skeletal muscle Methods: 40 wistar rats (age: 8 weeks) were randomly divided into 5 group: resistance training (R), endurance training (E), concurrent resistance-endurance training (R+E), concurrent resistance-endurance training (E+R), and control group (C). Training groups performed training program for 8 weeks and 5 sessions per week. Resistance training protocol consisted of 10 repetitions ladder climbing with load suspended from the tail between 30-80% individual maximum overload test. Endurance training protocol consist of treadmills run, with speed and duration of 9 m/min and 10 minutes in the first week to 30 m/min and 60 minutes in the last week.The concurrent training groups performed resistance and endurance training with opposite order .The FHL muscle of rats were removed at 24 hours after the last session of training and the levels of p-mTOR, p-p70S6K, p-AMPKα1 and MuRF-1 were measured by ELISA method. Results: The results showed p-mTOR was significantly greater in R and R+E training groups versus control group. A significant increase of p-p-70S6K was observed only in R training group. However, no significant difference between training groups was observed in p-AMPKα1 and MuRF-1. Conclusion: These results suggested that order of endurance or resistance exercise in a concurrent training program might influence on signaling pathways responses differentially and probably concurrent resistance-endurance training produce higher hypertrophic signaling responses compared to concurrent endurance-resistance training in long term. |
| Keywords |
| Keywords: concurrent training, mTOR, AMPK. |
| Status: Abstract Accepted (Poster Presentation) |
