| The effects of high intensity interval training and tryptophan supplement on proteins levels involved in skeletal muscle synthesis and atrophy in aging rats fed a high fat diet |
| Paper ID : 1276-SPORTCONGRESS |
| Authors |
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Mohadeseh Zohrabi *1, Parvaneh Nazarali2, Rostam Alizadeh3, Najmeh Rezaeinezhad4 1.Department of Exercise Physiology, Faculty of Sport sciences, University of Alzahra,Tehran,Iran 2Department of Sport Physiology , Faculty of Sport Sciences, Alzahra University, Tehran, Iran 3Department of Exercise Physiology,Sport Sciences Research Institute,Tehran,Iran 4Department of Exercise Physiology, Faculty of Sport sciences and Health, University of Tehran,Tehran,Iran |
| Abstract |
| Abstract Introduction: In this study we aimed at exploring the effects of high-intensity interval training (HIIT) in combination with tryptophan supplementation on genes expression of atrogin-1 and mammalian target of rapamycin (mTOR) in high-fat diet (HFD) fed rats Methods: To this end ,36 aged rats (54weeks old) were divided into five groups including normal diet sedentary, HFD sedentary, HFD+aerobic training, HFD+ tryptophan, HFD+aerobic training+tryptophan. Aerobic interval training was included 3 times a week for 8 weeks. Rats were consumed 100 mg/kg body weight of tryptophan daily. 48 hours after the last training session, after anesthesia, gastroqunemous was dissected and used for analyzing atrogin-1 and mTOR genes expression by Real time PCR method Results: The results showed that HFD increased atrogin-1 and mTOR genes expression in the skeletal muscle of aged rats in comparison with the normal-diet fed rats. In addition, HIIT decreased mTOR gene expression nonsignificantly in compared to the HFD sedentary group. However, HIIT decreased mTOR gene expression significantly in compared to the tryptophan supplementation with and without HIIT. Furthemore, atrogin-1 gene expression was lower in the HIIT group compared to the HFD sedentary, HFD+tryptophan and HFD+HIIT+tryptophan groups . Conclusion: In conclusion, HFD induced increase in gene expression of atrogin-1 and MTOR in aged rats, but HIIT reversed HFD effects |
| Keywords |
| : Aerobic Training, Tryptophan Supplementation, Hypertrophy, Skeletal Muscle, Muscle Atrophy, High-fat Diet |
| Status: Abstract Accepted (Oral Presentation) |
