| Cross-talk between SOCS3 gene expression and the antioxidant enzyme SOD in obese male mice following aerobic training |
| Paper ID : 1299-SPORTCONGRESS |
| Authors |
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masoumeh mohamadkhani1, Mandana Golami *1, Heshmatolah Parsian2, Hosein Abednatanzi1 1Department of Physical Education and Sport, Sciences,SR.C., Islamic Azad University, Tehran, Iran. 2Department of Physical Education and Sport Sciences, shahr-e-Qods Branch, Islamic Azad University, Iran. |
| Abstract |
| Introduction: In the present study, the changes and correlation between the antioxidant enzyme superoxide dismutase (SOD) and the expression of the cytokine signaling inhibitor gene (SOCS3) were examined in obese male mice. Methods: In this experimental study, 24 male Wistar rats were divided into three groups (eight in each): healthy control, high-fat diet (HFD), and HFD + aerobic training )Tr(. The rats in the HFD group received a special high-fat diet for 2 months prior to the main protocol. The rats in the aerobic training group ran on a treadmill designed for rodents for 6 weeks, five days a week. To assess the induction of fatty liver based on the implemented high-fat diet, the livers of the rats were examined using a Zonecare-Q9 ultrasound device before the start of the training protocol. Finally, the rats were sacrificed after the training protocol, and the SOCS3 variable was measured using Real-Time PCR, while SOD was measured using ELISA. For data analysis, one-way ANOVA and Pearson correlation methods were used. Results: The results obtained from the ultrasound showed that the high-fat diet in the HFD group induced grade 2 fatty liver compared to the healthy control group. The findings of the present study indicated that the HFD and HFD+Tr groups had a significant reduction in SOD compared to the healthy control group (p<0.05). Compared to the HFD group, the HFD+Tr group showed a significant increase in SOD (p<0.05). Additionally, the HFD+Tr group demonstrated a significant decrease in SOCS3 compared to the HFD group (p<0.05). However, the correlation between SOD and SOCS3 was not confirmed (p>0.05). Conclusion: Aerobic training increased SOD levels and reduced SOCS3 gene expression in obese rats, helping to modulate oxidative stress and inflammation; however, no significant correlation was observed between them. |
| Keywords |
| SOCS3, Exercise, SOD, NAFLD |
| Status: Abstract Accepted (Poster Presentation) |
