| EFFECT OF HIGH-INTENSITY INTERVAL TRAINING AND MODERATE-INTENSITY CONTINUOUS TRAINING ON P53 AND CYTOCHROME C LEVELS IN CARDIAC TISSUE OF OBESE TYPE 2 DIABETIC RATS |
| Paper ID : 1361-SPORTCONGRESS (R1) |
| Authors |
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هادی گلپسندی *1, محمد رحمان رحیمی2 1پژوهشگر پسادکتری، گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران. 2دانشیار فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران. |
| Abstract |
| Introduction: Type 2 diabetes with obesity causes cardiovascular damage via mitochondrial apoptosis and DNA damage pathways, involving key proteins p53 and Cytochrome c. This study compared the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on these proteins in the cardiac tissue of obese type 2 diabetic rats. Methods: The experimental study was conducted on 40 male obese Wistar rats (type 2 diabetes induced by a 12-week high-fat diet + 35 mg/kg streptozotocin injection). Rats were divided into four groups: diabetic control (DC), HIIT, MICT, and sham. The HIIT protocol consisted of 8 weeks, 5 sessions per week, at 85–95% VO₂max (1-minute high-intensity bouts + 1-minute recovery), while MICT was performed at 60–70% VO₂max for 40 minutes. Post-intervention, cardiac tissue was homogenized, and levels of p53, cytosolic Cytochrome c, and cleaved PARP were measured via spectrophotometry (ELISA Reader). Statistical analysis was performed using ANOVA and Tukey's test (P<0.05). Results: The results demonstrated that both HIIT and MICT significantly reduced apoptosis-related proteins. The p53 protein levels decreased by 51% in the HIIT group compared to the DC group (P<0.001), while the MICT group showed a 32% reduction (P=0.004). Cytosolic Cytochrome c, indicative of mitochondrial release, decreased by 55% in HIIT (P<0.001) and 38% in MICT (P=0.002). Cleaved PARP, an indicator of apoptosis execution, was reduced by 47% in HIIT (P<0.001) and 29% in MICT (P=0.01). Inter-group comparisons revealed HIIT’s superiority over MICT in reducing all three apoptotic proteins (P<0.05). Conclusion: HIIT exhibits a stronger protective effect against cardiac apoptosis in obese type 2 diabetic models compared to MICT, primarily through reduced p53 expression and inhibition of Cytochrome c release. Precise measurement of these proteins via spectrophotometry enables non-invasive and reproducible monitoring. These findings advocate for HIIT as a potential therapeutic intervention to prevent diabetic cardiomyopathy. |
| Keywords |
| High-Intensity Interval Training, Moderate-Intensity Continuous Training, Cardiac Apoptosis, p53, Cytochrome c, Type 2 Diabetes. |
| Status: Abstract Accepted (Poster Presentation) |
