| The Acute Effects of a Swimming Session and consumption of the active ingredient saffron on the expression of cardiomyocyte apoptotic genes expression in rats |
| Paper ID : 1429-SPORTCONGRESS |
| Authors |
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Sepideh Poursadeghi1, Fereshteh Shahidi1, Majid Kashef1, Elham Vosadi *2 1Department of exercise physiology, Faculty of Physical Education and Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran 2Department of exercise physiology, Faculty of Physical Education and Sport Sciences, Shahrood University of Technology, Semnan, Iran |
| Abstract |
| Introduction: Oxidative stress-induced cell death is activated by free radicals; Exercise and nutrition appear to have the potential to modulate cell proliferation and death. The aim of this study was to evaluate the acute effects of a swimming session and consumption of the active ingredient saffron on the expression of cardiomyocyte apoptotic genes expression in rats. Methods: In this experimental study, 32 male Wistar rats with average weight 180-200 g were randomly divided into four groups (1. oxygenated water, 2. oxygenated water + active ingredient saffron, 3. oxygenated water + exercise, 4. oxygenated water + exercise+ active ingredient saffron). After two weeks of familiarity with the environment and learning the training, all groups were injected 1 mM H2O2 and two groups 12.5 mg of active ingredient saffron peritoneally, and exercise groups The exercise groups swam only one session. After the exercise session, the rats were anesthetized with CO2 and their heart tissue was isolated. Real time PCR was used to measure cardiomyocyte apoptotic genes (Apaf-1, cytochrome c, and Caspase 9 genes) and to analyze the data, one-way analysis of variance analysis and skeletal follow-up test were used at the significant level of P ≤ 0.05. SPSS version 23 was used. Results: The findings showed that one swimming session, active ingredient saffron supplementation as well as interaction of one swimming session and active ingredient saffron supplementation reduced the germs of apoptosis, which included Apaf-1, cytochrome c and caspase 9 at a significant level P ≤ 0.05. Conclusion: Therefore, it is recommended to use saffron medicinal plants to reduce the adverse effects of cardiac apoptosis, reduce cell damage, and ultimately improve the health of the body after an acute exercise session. |
| Keywords |
| Active ingredient saffron, cardiomyocyte apoptotic genes, Swimming |
| Status: Abstract Accepted (Poster Presentation) |
