| The Effect of Eight Weeks of Aerobic Exercise on Inflamtory facrors in the Hippocampus of Epileptic Rats |
| Paper ID : 1432-SPORTCONGRESS |
| Authors |
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محمدرضا کردی *1, Amir Hossein Saffar Kohneh Quchan2, خدیجه عبادی زال3, سیروس چوبینه1 1استاد دانشگاه تهران 2Assistant Professor 3دانشجو دکتری دانشگاه تهران |
| Abstract |
| Introduction: Epilepsy is a chronic neurological disorder and the fourth most common neurological disorder in the world, in which a person experiences two or more seizures without a known cause during their lifetime. Inflammation is one of the key factors involved in the exacerbation of epilepsy, increased neuronal excitability, and the onset of seizures(1). The aim of this study was to investigate the effect of eight weeks of moderate-intensity aerobic training on the levels of MRP8, TNF-α, and IRAK4 proteins in the hippocampus of epileptic rats Methods: In this study, 32 male Wistar rats (4–6 weeks old) an average weight of 226.62±14.96 were divided into four groups: control(n=8), sham(n=8), epilepsy(n=8), and epilepsy + moderate-intensity aerobic training(n=8). After one week of the animals' adaptation to the laboratory environment, epilepsy induction was performed by intrahippocampal injection of kainic acid at a dose of one microgram using the stereotaxic method. To assess the success of epilepsy induction, the Racine Behavioral Scale was used. The exercise group performed moderate-intensity aerobic training for 40 minutes per session, five days a week, for 8 weeks(2). At the end of the training period, 48 hours after the last session, the rats were anesthetized using a combination of ketamine and xylazine, and their hippocampal tissues were extracted. Protein levels of MRP8, TNF-α, and IRAK4 were measured using the Western blot method. Results: Moderate intensity Aerobic training significantly reduced the hippocampal levels of IRAK4 (P<0.0001, F=89/77), MRP8 (P<0.0001, F=184/00), and TNF-α (P<0.0001, F=60/57) in epileptic rats. Aerobic exercise also significantly reduced the number of seizures in epileptic rats (P=0.01, t=2.80). Conclusion: Regular aerobic exercise can effectively reduce neuroinflammation and improve epileptic conditions by lowering the hippocampal levels of inflammatory markers MRP8, IRAK4, and TNF-α. |
| Keywords |
| Inflammation, seizures, regular exercise, central nervous system |
| Status: Abstract Accepted (Poster Presentation) |
